Microsatellite markers for the red band needle blight pathogen, Dothistroma septosporum.
نویسندگان
چکیده
Twelve microsatellite markers were developed for population analyses of the fungal pathogen, Dothistroma septosporum. Intersimple sequence repeat polymerase chain reaction (ISSR-PCR) and an enrichment protocol (fast isolation by amplified fragment length polymorphism of sequences containing repeats [FIASCO]) were both used to identify 28 unique microsatellite regions in the genome. From 22 primer pairs designed, 12 were polymorphic. These markers, screened on two populations representing 42 isolates, produced 40 alleles across all loci with an allelic diversity of 0.09-0.76 per locus. Cross-species amplification showed variable success with Dothistroma rhabdoclinis and Mycosphaerella dearnessi and some sequence variation within isolates of Dothistroma pini. These markers will be used to further study the population structure and diversity of D. septosporum.
منابع مشابه
Nested polymerase chain reaction-based detection of Dothistroma septosporum, red band needle blight of pine, a tool in support of phytosanitary regimes.
Red band needle blight is one of the most important foliar diseases of Pinus species and is of increasing international forest health and biosecurity concern. To provide a rapid identification technique for this pathogen in support of official control measures, a nested polymerase chain reaction-based diagnostic assay that employs species-specific primer sets has been developed. The assay is ab...
متن کاملGenetics of Dothistromin Biosynthesis of Dothistroma septosporum: An Update
Dothistroma needle blight is one of the most devastating fungal pine diseases worldwide. The disease is characterized by accumulation in pine needles of a red toxin, dothistromin, that is chemically related to aflatoxin (AF) and sterigmatocystin (ST). This review updates current knowledge of the genetics of dothistromin biosynthesis by the Dothistroma septosporum pathogen and highlights differe...
متن کاملSubstantial heritable variation for susceptibility to Dothistroma septosporum within populations of native British Scots pine (Pinus sylvestris)
The threat from pests and pathogens to native and commercially planted forest trees is unprecedented and expected to increase under climate change. The degree to which forests respond to threats from pathogens depends on their adaptive capacity, which is determined largely by genetically controlled variation in susceptibility of the individual trees within them and the heritability and evolvabi...
متن کاملHas Scots pine (Pinus sylvestris) co‐evolved with Dothistroma septosporum in Scotland? Evidence for spatial heterogeneity in the susceptibility of native provenances
Spatial heterogeneity in pathogen pressure leads to genetic variation in, and evolution of, disease-related traits among host populations. In contrast, hosts are expected to be highly susceptible to exotic pathogens as there has been no evolution of defence responses. Host response to pathogens can therefore be an indicator of a novel or endemic pathosystem. Currently, the most significant thre...
متن کاملEffector identification in the pine pathogen Dothistroma septosporum
Effectors are molecules produced by plant-associated organisms, such as fungal pathogens, that facilitate infection of specific plant hosts. Some effectors directly or indirectly suppress host defence responses while others help to mask the foreign invader from recognition by the plant. Plants can also recognise and respond to specific effectors to mount a resistance response. Thus the study of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Molecular ecology resources
دوره 8 5 شماره
صفحات -
تاریخ انتشار 2008